As EICAR.org is no longer providing eicar file through HTTP. You can test your antivirus provider from here:
Alternatively, you can also download ECIAR test sample from:http://rexswain.com/eicar.html
The Anti-Malware Testfile
This test file has been provided to EICAR for distribution as the „EICAR Standard Anti-Virus Test File“, and it satisfies all the criteria listed above. It is safe to pass around, because it is not a virus, and does not include any fragments of viral code. Most products react to it as if it were a virus (though they typically report it with an obvious name, such as „EICAR-AV-Test“).
The file is a legitimate DOS program, and produces sensible results when run (it prints the message „EICAR-STANDARD-ANTIVIRUS-TEST-FILE!“).
It is also short and simple – in fact, it consists entirely of printable ASCII characters, so that it can easily be created with a regular text editor. Any anti-virus product that supports the EICAR test file should detect it in any file providing that the file starts with the following 68 characters, and is exactly 68 bytes long: